ImagePro>2 linked classes of objects

[Catherine Cheniclet <catherine.cheniclet[at]bordeaux.inra.fr>]

Hello everybody,

        

I am
trying to work with two classes of objects in an image and I have
different kinds of trouble.

1)      I define the objects of the first class with the "automatic bright objects" option of the count/size command, then I would like to define the objects of the second class by using the "manual range selection". But by doing this it seems that I lose the 1^st class objects. In “measurement data” I can view only the data of one class or the other but not both at the same time.

2)      After having defined the second class objects (with manual range selection), I want to add some objects by drawing AOIs and converting them to objects. But they are converted into first class objects, and I don’t see where I could indicate that I want them to be objects of the second class.
3)      The third kind of problem concerns the labelling of objects. My objects are biological cells and each cell contains a nucleus. I want to know for each of them the area of the cell and the area of its nucleus. If I define two classes of objects (one for cells and one for nuclei), they are labelled independently and I have to make manually the correspondance between the area of a cell and the area of its nucleus (which has got a different number). Is there a way of doing this with IPP ?
        I imagine there are many applications where the relations between objects have to be taken into account.

Thank you very much if someone can help,
                                                                        Catherine

Catherine CHENICLET

Laboratoire de Biologie Cellulaire et Moléculaire du Développement des Plantes (BCMDP)

IBVM, UMR PBV 619, centre INRA de Bordeaux

71 avenue Edouard Bourleaux, BP 81

33883 VILLENAVE D'ORNON CEDEX           France

tél : (33) 05 57 12 25 60       fax : (33) 05 57 12 25 48