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Hi again,
Thanks to Bill and David for  trying to help me but  I am afraid that I cannot find the optical the IOD (integrated optical density ) function . There is nothing in the manuals or help menu.
Attached is an image with will demonstrate specifically what I am trying to achieve and indeed did so with ease in Optimas. It is a histological section which has been stained with  a specific marker that has a variable affinity across the section.
I want to measure optical density along the curved red line at the points defined by  y, and at  the intervals of x .
This was easily done in Optimus (luminescence anyway) and the parameters of x and y were easily changed.
Image pro line profile is sort of on the right track but a little difficult to control but with help from you lot out in cyberspace I am sure it will be overcome ,after all it was a large outlay if we have gone backwards on functionality of software.
ta Michael

Michael Archer
Dept of Zoology
University of Western Australia




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