Features

• Acquire files directly from camera, scanner, disk, or CD-ROM
  
• Streamlined capture interface 
  
• Get an optimized view with dynamic auto-range
  
• User-defined live scaling for low-light acquisition
  
• AutoSet feature takes the guesswork out of setting exposure parameters
  
• Semi-transparent live preview feature assists in tiling, stitching, and aligning images
  
• Save and change settings based on camera or application with a single click
  
• Capture multiple images after user-specified delay/acquisition time
  
• Acquire, create, and playback a sequence (stack) of images
  
• Apply filters or operations to individual frames or to entire sequence with sequencer playback option
  
• Includes capture support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit gray scale input devices

Learn more about our Windows 7 support and enhancements in Image-Pro Plus & Analyzer 7.0.1.

Memory Management

Image Processing Tools 

• Stitch and tile images 
  
• Automatic alignment of image stacks to correct for stereo, rotational, and other types of shifts
  
• N-dimensional viewer lets you choose variables to display from image sets
  
• Create in-focus composite images from partially in-focus source image stacks with EDF (Extended Depth of Field)
  
• EDF Test Strips automatically tests all EDF options on your images with a single click
  
• Set Manager allows you to easily organize, extract, and navigate multiple image sets
  
• Dye Manager lets you apply dyes to gray scale images by choosing emission wavelength
  
• Local Zoom tool allows you to simultaneously view your entire image in one window, while viewing a more magnified area of that image in a second window
  
• Work with topographic maps for composite in-focus images
  
• Create composite in-focus images with maximum local contrast, maximum through depth contrast, maximum intensity and minimum intensity
  
• Create composite images from an infinite number of source gray scale images with the Color Composite Tool
  
• Use the Sequence Gallery to produce a montage image that allows for preview of all image analysis processes on image stacks
  
• Perform spatial and logical arithmetic, as well as image alignment operations
  
• Combine images with Image Overlay
  
• Create, print, or save a collage of several images with Mosaic Image
  
• Combine the results of two segmentation algorithms with Restricted Dilation Feature
  
• Create grids for intercept counting
  
• Use Image Registration tools for comparative analysis

Image Enhancement Tools

• Edit image intensity level with Display Range
  
• Work with background subtraction and correction
  
• Manually or automatically enhance color and contrast using equalization, gamma correction, contouring, or thresholding
  
• Invert, reset, and apply contrast to images
  
• Reverse pixel values with automatic image inversion

Image Filtering

• Work with Filter dialogs that include descriptions and interactive preview windows
  
• Use Enhancement filters that include Low-Pass, Hi-Pass, Gaussian, Hi-Gaussian, Sharpen, Flatten, Median, Rank, Local Equalization, and Despeckle in user-definable kernel sizes
  
• Outline objects with various Edge Filter methods including Sobel, Roberts, Laplace, Variance, Phase, Horizontal, and Vertical
  
• Process objects with Morphological filters including Erode, Dilate, Open, Close, Top Hat, Well, Branch/End, Watershed, Thinning, Pruning, Distance, and Reduce in user-definable kernel sizes
  
• Work with Large Spectral Filters: Fast-acting, large kernel size (up to 4000 x 4000) filters that include Low-Pass, Hi-Pass, Band-Pass, and Edge filters
  
• Apply filters to individual frames or to entire sequence
  
• Customize kernel files for morphological and convolution filters

Fast Fourier Transform (FFT)

• Create forward and inverse transforms
  
• Edit the spectrum directly including Low-Pass, High-Pass, unsharpen, spike cut, and spike boost
  
• Save and reload data

Fluorescence Lookup Tables and Overlays

• New feature to apply tint to gray scale images automatically
  
• Extract and merge multiple color channels (including RGB, HSI, HSV, and YIQ) simultaneously
  
• Use color information to separate objects from background
  
• Segment image based on the color channel histogram or color cube
  
• Set pre-defined and custom color spreads (ranges) with the Pseudocolor tool

3D Imaging Tools (Requires Open GL graphics card)

• Voxel-based 3D filters for use with 3D volumetric, time-lapse and other 3D image sets 
  
• Built-in Open GL viewer for interactive 3D volume rendering

SharpStack Deconvolution Tools

• Built-in real-time 2D deconvolution
  
• Built-in Nearest Neighbor and No-Neighbor deconvolution

Spatial and Intensity Calibration

• Calibration wizard helps you to calibrate an active image and create reference and system calibrations
  
• Use X and Y scale bars as a nondestructive overlay
  
• Work with intensity or spatial measurements
  
• Create and display spatial calibration markers
  
• Create and display system calibrations
  
• Use pre-defined spatial calibration units
  
• Save and recall all calibrations
  
• Calculate derived calibrations automatically
  
• Store information about your microscope in lens information files

Classify Objects

• Count and Size objects automatically
  
• Measure areas, perimeters, lengths, roundness, major and minor axes, aspects, angles, centroids, holes, population density - over 50 measurements
  
• Identify over 100,000 objects per frame (memory dependent)
  
• Set ranges for measurements to filter unwanted objects
  
• Group objects in classes based on measurements
  
• Resolve clustered objects with Watershed, Auto-Split, and Cluster analysis tools
  
• Use colors to separate and measure objects in color images
  
• Display measurements as histograms and scattergrams
  
• Manually tag, count, and classify objects
  
• Work with Auto Threshold
  
• Includes option to manually tag, count, and classify objects

Object Tracking

• Tracking tools allow you to follow objects in a sequence image and measure distance traveled manually, automatically, and semi-automatically
  
• Correctional tracking allows you to track objects when image segmentation is difficult or not possible
  
• Static & Dynamic Intensity tracking allows you to track and graph the changing of intensity parameters over time within an area of interest
  

Spatial Measurements

• Snap feature allows you to automatically save measurments into images for documentation
  
• Scale bar for X and Y measurements can be placed on your image as an overlay
  
• Measure co-localization of probes in seperate gray scale images or within a color image
  
• Work with weighted centroid, clumpiness, heterogeneity, min/max density, dendrite length, end point, fractal dimension, margination, and additional perimeter measurments
  
• Record Pass/Fail routines in a macro and play back when needed
  
• Measure best-fit line, arc, and circle with metrology tools
  
• Outline objects automatically with Auto-Trace feature
  
• Measure lenghts, areas, perimeters, and angles
  
• Calculate max,min, and average thickness between lines
  
• Use the Caliper Tool for edge detection and measurement
  

Image Analysis

• Visualize image data with scattergrams, histograms, and line profiles
  
• Measurement equation editor 
  
• Export data via DDE to Origin® or Microsoft® Excel
  
• Dynamically link to overlays in an image
  
• Color management tools to manage inputs and outputs for your entire imaging system to achieve true color fidelity
  
• Display "Dark Mode" for low light laboratory environments
  
• Display image intensity values in a 3D Plot with Surface Plot
  
• Collect data from multiple images with Data Collector
  
• Live data collecting and charting
  
• Calculate straight line, circle, irregular line, or area histograms
  
• Use new thickness measurement options
  
• Analyze percent area of multiple threshold levels
  
• Define and manage multiple areas of interest (AOI) in a single image
  
• Analyze RGB, HSI, HSV, or YIQ content of color images
  
• Combine image with background correction for precise intensity or optical density measurements
  
• Display background corrected line profiles
  
• Output intensity map in ASCII format

Image Annotation

• Work with non-destructive image annotation
  
• Add text, simple graphics, and arrows
  
• Modify your palette or colors

Image Database

• Use built-in database or archive, manage, and extract knowledge from your images with the complimentary 6-month trial IQbase 2.0 Image Database
  
• Locate images through keyword searches or view in a thumbnail gallery
  
• Print full-size or image galleries from inside the database
  
• Batch process multiple discreet images
  
• Perform SQL multi-parameter searches

Report Generator

• Create customized reports with images, data, and text
  
• Create and save report templates

Printing and Publishing

• Print frames from a sequence or montage of single images with the Mosaic printing feature
  
• Output gray scale and color images to Windows® printers
  
• Maintain complete control over image position on your page
  
• Print tiles to make poster size prints
  
• Use the Test Strip feature for selection of best output
  
• Screen capture individual images or entire screen

Internet Support

• Send e-mail messages within Image-Pro Plus
  
• Review, send, and receive data over the Internet using IRC (Internet Relay Chat)

Customization & Macro Programming 

• Floating workflow toolbar builder allows you to create unique toolbars for your specific work process
  
• Built-in configurations customize Image-Pro Plus for your industry needs, life science or industrial
  
• Customize your application environment with the interactive menu editor
  
• Demo macro browser includes updated running examples
  
• Use expanded example code in your own macros
  
• Use the IPBASIC engine with debugger, full editor, and dialog builder
  
• Record sessions and play them back in Basic Syntax
  
• Assign scripts to files and function keys
  
• Add basic loops and conditionals
  
• Macro editor upgrades, including command recognition
  
• Use with COM objects such as Microsoft® Word or Excel
  
• Integrate and customize macros with Microsoft® Visual Basic® or Visual C++®
  
• Work with the Dialog Box Editor

Image Data and File Format Support

• Read TIFF, IPW, JPEG, JPEG2000, EXIF JPEG Flat (binary), TGA, BMP, PhotoCD, PICT, CUT, PCX, GEL, PCT, and HDF
  
• Write and convert files to TIFF, IPW, JPEG, JPEG2000, Flat, TGA, BMP, PICT, PCX, and EPS
  
• Stack and Confocal read file support for SEQ (Image-Pro Sequence), STK (Metamorph Stack), PIC (Biorad confocal), LSM (Zeiss confocal), DEB and AVZ (Autoquant Stack), LEI (Leica Confocal), QED, Fluoview 1000 and DM3 (Digital Micrograph)
  
• Read and write SEQ and AVI files
  
• Support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit gray scale as well as 32 bit floating point images
  
• JPEG, LZW, and RLE compression supported
  
• Batch convert files
  
• Output data to ASCII, WK1, or XLS for input for spreadsheets
  
• Transfer images, data graphs, and data files via DDE or Clipboard
  

 
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