• 3D Image Analysis Software IMAGE-PRO PREMIER 3D
    3D Image Analysis Software IMAGE-PRO PREMIER 3D

    Powerful 3D Image Analysis Software

    3D image analysis software designed for research professionals who need to view and measure structures in a variety of spatial or temporal image dimensions.

    Image-Pro Premier 3D is unique because it delivers all the essential 3D analysis tools in one easy-to-learn and use software package.

     

    Measure automatically

    • 3D Size and Shape
    • 3D Object Count
    • 3D Intensity and Density
    • Size and Shape

      Calculate 3D object and region dimensions, ferret sizes, volumes, and more. Know everything there is to know about the morphology of you image materials.
    • Object Count

      Quantify the 3D objects in your volume with ease. Account for touching objects or even partial objects that have been severed by the volume boundaries.
    • Intensity and Density

      Determine how bright or dark your 3D objects are one-by-one or in total with measurements such as Integrated Optical Density.

    Identify what to measure

    • Segment by Intensity in 3D
    • Filter by Measurement in 3D
    • Classify by Measurement in 3D
    • Segment by Intensity

      Use histogram-based methods to select your areas of interest, instantly highlighting them as 3D objects in the volume display. There is no limit to the number of segmentations you can perform, so even subtle variations in gray-scale or color to be separated for analysis.
    • Filter by Measurement

      With any of the chosen measurements, create a histogram of your segmented regions and only keep what you find relevant, excluding the rest using multiple precise parameters such as a combination of morphology and intensity measurements.
    • Classify by Measurement

      Using an automatic classification method, bins of classes can be created and applied to all the measured 3D objects in the data graph. Use this to easily color-code and classify 3D objects in the view and on the data tables and graphs.
    Object Splitting & Merging
    It becomes necessary to split touching objects in many images so we’ve provided both automatic watershed-based splitting techniques as well as a manual point-to-point based splitting method to get the job done.

     

    Object naming and coloring
    This makes it easier to keep track of what’s what by allowing each object to receive a unique name and color through editing the data table or simply double-clicking the object itself.

     

    Eliminate objects touching image border
    In cases where you only want complete and intact volumes that are not cut off by the image stack’s borders you can enable a clean borders setting to ignore these objects.

     

    Object appearance
    The appearance of each object is very important to accurate visualization so parameters for each object are able to edited including color, transparency, specularity.

     

    Guided Segmentation

    Measure complex 3D objects

    1
    Generate Outlines
    Use any 2D frame to define objects as unique collections of outlines. Name and color code them for clarity.
    Generate Outlines
    2
    Z Propagation
    Propagate the segmentation up or down through the stack, using Auto or Semi-Auto techniques and review the outlines for edits as needed.
    Z Propagation
    3
    Isosurface Creation
    Automatically generate isosurfaces from your collection(s) of outlines. Measure volume, surface, intensity, and unique morphological object measurements.
    Isosurface Creation
    Guided Segmentation enables a whole new world of ultrastructure imaging by tackling the most difficult 3D image analysis challenges, employing a simple guided workflow.

    Manual 3D measurements

    Measure precisely what you want

    Measure length along surface
    Length along surface
    Length from surface to surface
    Length from surface to surface
    Circumference
    Circumference
    Coordinates
    Coordinates
    Distances from reference point
    Distances from reference point
    Angle from reference plane
    Angle from reference plane
     

    Retain quantitative accuracy

    Calibrated voxels
    Calibrated voxels based on XY spatial calibrations and Z spacing calibration
    Sub voxel accuracy
    All measurements are based on precise sub voxel accuracy

    3D/4D Data Management

    Make the data work for you

    3D/4D Data Management
    Choose from 60+ measurements to display in tables and graphs
    Rename and adjust
    Rename objects by clicking and typing in the table. Also arrange all objects by color coded class to see relationships.
    Rename and adjust
    Sort and Condense
    Sort and Condense
    Sort by measurements and adjust tables to only show the relevant data.
    Group and view statistics
    Combine classes of similar objects into a custom hierarchy and see statistics per measurement for each grouped class.
    Group and view statistics

    Data Collector

    Gather data from multiple experiments

    • Collect data automatically
    • Keep data linked to images
    • Graph the results
    • Collect data automatically

      With the press of a button all the data from your table is compiled into Data Collector. You can even set it up to collect from all images automatically as they are measured.
    • Keep data linked to images

      For a multi-image experiment, collect large clusters of data from each image separately and retain the original links to understand how image groups compare and contrast.
    • Graph the results

      Use a variety of graph types for your data such as 2D and 3D scatterplots, histograms, block charts and lines graphs.
    Export Data
    Export your data and statistics to Microsoft Excel, File, or the Clipboard for pasting anywhere on your PC.
    Send data to reports
    Send your data and screenshots to custom designed reports within Image-Pro for fast and easy creation of experiment results.

    3D/4D Visualization

    Exceptional visualization made easy

    • 3D/4D view
    • Slicer view
    • Gallery view
    • Synced Comparison
    • 3D/4D view

      Visualize your 3D volumes or 4D time-lapse data in stunning high resolution full screen views using this mode.
    • Slicer view

      Explore every voxel using this 2D projection mode that links the position of the 2D plane to a real 3D position on the integrated 3D view.
    • Gallery view

      See every frame that makes up the volume in a gallery of thumbnails able to be scaled, edited, adjusted or simply viewed in this mode.
    • Synced Comparison

      Sync any two data sets for side-by-side viewing, zooming, and panning. Sync a 3D view a slicer or two of the same.

    Control what you see

    Volume adjustments
    Volume adjustments
    • Blend, MIP, and Sum Projections
    • Overall Volume Transparency
    • Wireframe & Isosurface Renderings
    • Slice Density and Edge Coloring
    • Pseudo-Surface and Stereo Views
    Object parameters
    Object parameters
    • LUT-based Spread and Opacity
    • Individual object Specularity
    • Color Palette Editor & Presets
    • Light Sources and Shadows
    Channel controls
    Channel controls
    • Retain native channel coloring
    • Toggle or Blink each channel
    • Opacity weighted auto blending
    • Independent histogram edits
    • Channel extraction or Merging
     
    Change Backgrounds
    Change Backgrounds
    Change your background colors and apply gradient blending to create the highest possible contract between your data and the background.
    Apply Shadows
    Apply Shadows
    Shadow Projections can produce shadows on 3 walls of the volume frame and accentuate the morphology and interaction of objects.
    Adjust Lighting
    Adjust Lighting
    Control your volume’s light in two different directions with intensity, color, and angle adjustments.
    Remove Regions
    Remove Regions
    View the interactions of various materials by clipping away distracting or unnecessary regions using a Volume of Interest.
     

    Enjoy your stunning results

    Enjoy your stunning results

    3D Clipping Planes

    Clip. Discover. Repeat.

    Ortho clipping planes
    Create any number of orthogonal planes facing the X, Y, or Z axes and easily traverse the axis of orientation. Reveal what a 2D plane in any position looks like or turn clipping on to erase data before or after the plane.
    Ortho clipping planes
    Oblique clipping planes
    Add oblique planes at any orientation in the volume to investigate the internal structure of 3D objects that are not obvious using standard slicer or ortho planes.
    Oblique Clipping Plane
     
    Drag to adjust priority
    Drag to adjust priority
    Select any clipping plane in the 3D View Panel and move it above or below other clipping planes or 3D elements to adjust the clipping priority. Items on top clip all those below it, it’s as simple as that.
    Maneuver with handles
    Maneuver with handles
    Use these intuitive on-image handles to position planes in the exact position for optimal view or measurement. Additionally, similar controls are also available in the 3D View Panel for fine control.
    Measure between planes
    Measure between planes
    Use any two planes to measure distances at any angle. Using 2D planes gives you an advantage of precise placement and unique perspective unrivaled by standard 3D.

    3D Processing

    Make dataset corrections

    Alignment

    Align your stack using a variety of methods such as our automated Fourier Transform algorithm or an intensity-based algorithm for images where complex details change incrementally from frame-to-frame. When aligning stacks with repeating patterns or fiduciary marks, other options are available for alignment including a full-featured manual correction tool.

    Before Alignment
    Before Alignment
    After Alignment
    After Alignment

    Normalization

    Intensities can vary across individual planes and the entire stack from top to bottom. Set your active frame and normalize the entire stick in a single click.

    Before Alignment
    Before Normalization
    After Alignment
    After Normalization
     
    3D Filters
    3D Filters
    Apply a variety of 3D filters
    VOI Crop
    VOI Crop
    Crop the dimensions of the volume

    Key Frame Animations

    Share your data with movies

    Key Frame Animations

    Use path templates
    Use path templates
    • Circular animation
    • Fly-around animation
    • See-saw (Rock) animation
    • Custom animation path
    Fine-tune the timeline
    Fine-tune the timeline
    • Record unique camera positions
    • Add/Replace key frames
    • Add more frames for smoother movie play back selectively or across timeline
     
    Produce
    Produce
    Create high quality movies in WMV, AVI, and MP4 formats
    Save
    Save
    Save, Load and Copy Animation Paths and volume settings for use on future animations
     

    Watch Example Animations

    Explore what is possible using the key-frame driven animation.

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