• IMAGE-PRO PREMIER Heroshot
    IMAGE-PRO PREMIER

    2D IMAGE ANALYSIS SOFTWARE

    Capture, process, count, classify, measure and share.

    A powerful and customizable imaging platform driven by over 30 years of user feedback, the new Image-Pro Premier offers native 64-bit support, a user friendly interface and a suite of 2D measurement solutions.

    Rely on our worldwide network of Image-Pro partners, superb training tools, and technical support team to help you get started and keep your research moving forward.

     

    Automatic Measurements

    SEGMENT, COUNT/SIZE, CLASSIFY, and ANALYZE

    Image-Pro’s technique for performing Automatic Measurements is the foremost solution for gathering data from images by segmentation systems. Our simple step-wise approach to the problem is designed to provide the ultimate flexibility to analyze nearly any image type while remaining simple enough to quickly learn and teach to others.

    1 SEGMENT

    Identify what you want to measure

    Histogram-based Segmentation

    Use the method that best suites the image and identify what you want to measure

    • Select a single intensity range
    • Select multiple intensity ranges
    • Select with the picker
    • Select a single intensity range

      Use histogram-based methods to select your areas of interest, instantly highlighting them as objects on the image. For high contrast images use the Auto Dark and Auto Bright methods.
    • Select multiple intensity ranges (classes)

      Don’t stop at a single range, add any number of classes to segment any range of intensities, whether contiguous or spaced apart on the histogram, classes can be a great way to find various materials in an image.
    • Select with the picker

      This simple, but handy, tool enables you to use a range of sizes for the picking area under the cursor and select those pixels as the representative selection on the histogram for your segmentation.

    Smart Segmentation

    A revolutionary new method that employs a pixel classification algorithm able to identify hard to segment objects and regions. Use Smart Segmentation to identify faintly-colored objects, textured objects, and objects or regions on uneven backgrounds.

    • Select objects and background
    • Define the recipe parameters
    • Train over multiple images
    • Select objects and background

      Easily add object markers to the image to identify representative samples of your objects or regions and lastly add a background marker. Set up multiple object classes and even create select multiple regions per class to refine the representative pixels.
    • Define the recipe parameters

      Use a range of “recipe” options to segment, including Intensity, Color, Background and Morphological Filters. Once you have a recipe that fits your segmentation needs, you can save and reapply to other images. For more complex samples a custom option can pre-apply filters.
    • Train over multiple images

      Most experiments require the acquisition of many images from one or more samples. Smart Segmentation makes this analysis easy because the algorithm can be trained over a range of images in order to learn how your objects should be most accurately segmented.

    Smart Segmentation solves common problems.


    Automatically compensate for uneven backgrounds

    Automatically compensate for uneven backgrounds

    Select two regions, and Smart Segmentation will automatically calculate the difference in the unevenly illuminated background. This is normally very difficult with histogram-based methods as objects will typically be the same intensity as some areas of the background.

    Select & Classify Objects by Color

    Select & Classify Objects by Color

    Locate and segment objects based on their color. Create new classes to further characterize and streamline data collection and reporting. Creating classes is easy, and doesn’t have a limit to how many you can create, in order to make it easy to organize important objects.

    2 COUNT/SIZE

    Create object outlines, instantly counted and sized

    Filter by Measurement

    Choose from a wide number of measurements to filter the entire segmentation group from and apply specific range restrictions (graphically or numerically) that selectively leave your objects of interest for further classification and measurement.

    Area = ( 486 – Max )
    Objects in range = 210

     

    Filter out objects to be counted and sized by any number and combinations of parameters.

    Area = ( 1486 – Max )
    Objects in range = 57

     

    Reduce the segmented objects down to the proper number and then press COUNT/SIZE.

    Create object outlines.

    Once objects are identified and outlined you can count and measure areas, percent area, regions, intensity values, and more.

    Count Number

    Count Number
    All discrete, separate objects can be automatically counted within the set range of intensities.

    Size and Shape

    Size and Shape
    Automatically measure object area, percent area, perimeter, length & width, radii and feret ratio, etc.

    Intensity and Density

    Intensity and Density
    Automatically measure object intensity, integrated optical density, density, and intensity over time.

    Object Splitting & Merging
    It becomes necessary to split touching objects in many images so we’ve provided both automatic watershed and boundary shape-based splitting techniques as well as a manual point-to-point and polyline-based splitting methods to get the job done.

    Object naming and coloring
    This makes it easier to keep track of what’s what by allowing each object to receive a unique name and color through the editing of the data table. Change a single object or select a large number of objects and change them as a group.

    Eliminate objects touching image border
    In cases where you only want complete and intact volumes that are not cut off by the image stack’s borders you can enable a clean borders setting to ignore these objects.

    Fill Holes
    The appearance of each object is very important to accurate visualization so parameters for each object are able to be edited including color, transparency, specularity.

    3 CLASSIFY

    Separate objects into custom groups by parameter

    Single Variable Classification

    Single Variable Classification
    Define any number of classification bins, set the first and last values of each bin, and assign classes to objects according to the bin ranges. Use any parameter in the measurement list to classify the objects by that "single" variable.

    Auto Classification
    Define the number of groups to be created, choose all the measurement parameters to be used for the classification, and then apply hierarchical clustering to the objects. Classes will be assigned based on the cluster created by cutting the clustering tree where the number of branches correspond to the number of requested classes.

    Auto Classification
    Learning Classification

    Learning Classification
    Classify objects using multiple parameters based on manually selected reference objects. This is an especially useful technique when you are not certain which parameters to use for the classification, but have some idea of how the objects should appear per class.

    4 ANALYZE

    Generate data for making helpful comparisons

    Measure Distances Between Objects

    Measure Distances Between Objects
    Measure one-to-one and one-to-many distances between objects.

    Measure Objects within Objects

    Measure Objects within Objects
    Analyze parent/child relationships with tools that allow you to automatically measure and group objects within objects.

    Sort Counted Objects
    Create a new image displaying all counted objects arranged by size.

    Sort Counted Objects

    Analyze the Spatial Distribution of Objects

    Measure Objects within Objects

    Measure and Analyze

    Extract quantitative data with ease

    Image-Pro Insight has been designed for a diverse user base and a multitude of applications. Whether in the materials science research lab, the quality assurance room, or the manufacturing floor, Insight’s semi-automated measurement and editing tools enable accurate and expedient analysis.

    Measure and Analyze

    Calibrate for accuracy

    Calibration data is ideally read from the image, however is cases where it does not exist, we offer a variety of options to easily create and apply a calibration to an image

    Calibrate for accuracy

    Measure Layer Thickness

    Determine the top and bottom edges and allow the software to measure thickness and statistics about longest and shortest lengths.

    Measure Layer Thickness

    Measure Line Profile

    Draw a variety of lines and shapes for displaying the intensity under the lines as Line Profiles. Apply any number of line profiles and export the graph and data out to Excel or to a custom report.

    Measure Line Profile

    Measurement tools

    • Direct and Relative
    • Manual and Auto Tracing
    • Magic Wand Counting
    • Direct and Relative

      Extract accurate data from your calibrated images with a wide range of manual and semi-automated measurement tools.
    • Manual and Auto Tracing

      Draw polylines and regions to trace complex objects for you to measure area, perimeter, and length.
    • Magic Wand Counting

      One-click automatic segmentation of the local area based on the pixels clicked. Refine selection with ‘Add’ and ‘Remove’.
    Magnetic Snap Measurements
    Magnetic Snap Measurements
    Draw accurate line measurements every time with the Snap Measurement tools.  Simply draw and the measurement will accurately snap along object edges.
    Classify measurements
    Classify measurements
    Use different colors, shapes, and custom names to classify and organize your measurement data. Use to organize measurements for greater clarity.

    Data Management

    Make the data work for you

    3D/4D Data Management
    Choose from 40+ measurements to display in tables and graphs
    Rename and adjust
    Rename objects by clicking and typing in the table. Also arrange all objects by color coded class to see relationships.
    Rename and adjust
    Sort and Condense
    Sort and Condense
    Sort by measurements and adjust tables to only show the relevant data.
    Group and view statistics
    Combine classes of similar objects into a custom hierarchy and see statistics per measurement for each grouped class.
    Group and view statistics

    Data Collector

    Gather data from multiple experiments

    • Collect data automatically
    • Keep data linked to images
    • Graph the results
    • Collect data automatically

      With the press of a button all the data from your table is compiled into Data Collector. You can even set it up to collect from all images automatically as they are measured.
    • Keep data linked to images

      For a multi-image experiment, collect large clusters of data from each image separately and retain the original links to understand how image groups compare and contrast.
    • Graph the results

      Use a variety of graph types for your data such as 2D and 3D scatterplots, histograms, block charts and lines graphs.
    Export Data
    Export your data and statistics to Microsoft Excel, File, or the Clipboard for pasting anywhere on your PC.
    Send data to reports
    Send your data and screenshots to custom designed reports within Image-Pro for fast and easy creation of experiment results.

    Process and Enhance

    Prepare your images

    One-click Color Composite

    Build composite color images by simply right-clicking on each grayscale image to select a color tint.

    Lock playback multiple sequences

    Extract and Merge Channels

    Easily combine and separate images into RGB, HSI, and HSL color channels.

    Extract and Merge Channels
    Image Correction

    Image Correction

    Correct your image background to better distinguish image objects, improving the downstream measurement operations and reducing false positives.

    Image Filters

    Image Filters

    Remove background noise and reveal hidden details with an extensive set of image processing filters. Preview filters on your active image for instant results.

    HDR Post process

    Capture a sequence of images at different exposures, with variable options, and combine them into a single High Dynamic Range image. The resulting image is a compilation of image data using the widest possible range of dark to light pixels. No more black or saturated pixels.

    HDR Post process

    Alignment & EDF

    Correct for microscope shift with post-acquisition auto-alignment tools. Extended Depth of Focus (EDF) creates a focused image from a series of images captured at different z planes.

    Alignment & EDF

    Report and Share

    Prepare your images for what’s next

    Annotations

    Draw arrows, text annotations, meta-data overlays, Date/Time stamps, calibration marker bars and more.

    Annotations

    Quick Reports

    Send data and images to Insight’s report generator, able to create simple custom reports based on pre-defined templates. Print or export to Word or PDF.

    quick report2
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